Translational_Unit

Part:BBa_K329011

Designed by: Xavier Duportet   Group: iGEM10_Paris_Liliane_Bettencourt   (2010-10-17)


RBS + Xis-Int HK022

This part allows the expression of both the Excisionase and the Integrase of the double-stranded HK022 DNA enterobacteriophage of Escherichia coli, member of the lambdoid or lambda-like group of phages. Expression of these two proteins promotes the excision of the phage DNA by recombining the attR and the attL sites. After excision, the original attB and attP sites are reformed.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None